Part:BBa_K103016:Design

OmpA_linker_omega_linker

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 802
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 596
  

Design Notes

Preparation of BBa_K103016 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=15&arg0=18_September_2008&arg1=29_September_2008&arg2=30_September_2008&arg3=2_October_2008&arg4=3_October_2008&arg5=6_October_2008&arg6=7_October_2008&arg7=8_October_2008&arg8=13_October_2008&arg9=14_October_2008&arg10=15_October_2008&arg11=16_October_2008&arg12=17_October_2008&arg13=21_October_2008&arg14=22_October_2008&name=Preparation%20of%20OmpA-linker-omega-linker%20%28BBa_K103016%29 here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).

OmpA_linker_omega_linker fragment was amplified from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha pACYC177+OmpA-omega-ΔA-alpha] using primers: 5' ATTAGATCTGAATTCGCGGCCG CTTCTAGAGGGCATATGAAAGCTACTAAACTGGTAC 3' and 5' AGCCTGCAGCGGCCGCTACTAGTACCGGATCCCGAACCACCCCCACCCCCGCTACC 3'. Resulting product was digested with PstI and BglII and ligated into pACYC177 ([http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha pACYC177+OmpA-omega-ΔA-alpha] digested with PstI and BamHI)